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BIO BENCH

AccuPAGE™ Bis-Tris Precast Gel

AccuPAGE™ Bis-Tris Precast Gel

Regular price €29,00 EUR
Regular price Sale price €29,00 EUR
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 Documents

Datasheet - AccuPAGE™ Precast Gel

User Guide - AccuPAGE™ Precast Gel

Pay by bank transfer, or any question during purchase? Talk with a representative by email to hxinfo@helixarbio.com.

Bio Bench AccuPAGE™ protein electrophoresis gels are precast polyacrylamide gels that provide large range of molecular weight separation ability with good resolution and sample integrity. AccuPAGE™ provides 2 types of gels, including conventional Tris-Glycine gel and the Bis-Tris gel. These 2 types of gel are both optimized by Bio Bench, in order to provide better performance as well as good stability during long term storage.  AccuPAGE™ is compatible with nearly all of tanks from major biotech providers. Take out from the fridge and open the package and the sealing, then you can operate it under the routine lab instructions.

AccuPAGETris-Gly gel is made of optimized formula from conventional Tris-Glycine gel. It has good stability and long shelf life for up to 12 months. If you lab has been using Tris-Glycine gel, then you can shift to AccuPAGETris-Gly precast gel smooth.

AccuPAGEBis-Tris gel delivers consistent gel performance with a neutral pH environment (pH≈7.0) that minimizes any protein modifications. Bis-Tris can improve the stability of the gel structure during electrophoresis, which will avoid the gel become trapezoid shape and lead to sharp and straight band after staining. Your lab device does not need to be changed to run AccuPAGEBis-Tris gel – only need to remember Bis-Tris gel need to be run in MOPS or MES buffer.


AccuPAGE™ gel separation

 

How to use the AccuPAGE™ precast gel - a quick guide

 

 

 

AccuPAGE™ precast gel family

Gel type

 

 

Running Buffer

 

 

Gel Thickness

 

 

Gel Concentration

 

 

Max Separation

 

 

Recommended Separation

 

 

Well Amount

 

 

Max Sample Load

 

 

Cat. No.

 

 

Tris-Glycine

Tris-Glycine

1mm

6%

40-300kDa

60-200kDa

10

50μL

AP-GPG-610

15

30μL

AP-GPG-615

 

 

8%

25-300kDa

40-200kDa

10

50μL

AP-GPG-810

 

 

15

30μL

AP-GPG-815

 

 

10%

11-230kDa

20-160kDa

10

50μL

AP-GPG-1010

 

 

15

30μL

AP-GPG-1015

 

 

12%

6.5-200kDa

15-85kDa

10

50μL

AP-GPG-1210

 

 

15

30μL

AP-GPG-1215

 

 

15%

5-170kDa

10-50kDa

10

50μL

AP-GPG-1510

 

 

15

30μL

AP-GPG-1515

 

 

4% - 15%

5-270kDa

20-200kDa

10

50μL

AP-GPG-41510

 

 

15

30μL

AP-GPG-41515

 

 

4% - 20%

3.5-300kDa

5-200kDa

10

50μL

AP-GPG-42010

 

 

15

30μL

AP-GPG-42015

 

 

Tris-Bis

MOPS or MES

1mm

8%

8-270kDa

25-245kDa

10

50μL

AP-GPB-810

15

30μL

AP-GPB-815

10%

8-270kDa

11-180kDa

10

50μL

AP-GPB-1010

15

30μL

AP-GPB-1015

12%

3.5-245kDa

11-135kDa

10

50μL

AP-GPB-1210

15

30μL

AP-GPB-1215

4% - 12%

3.5-270kDa

11-245kDa

10

50μL

AP-GPB-41210

15

30μL

AP-GPB-41215

 

 

 

AccuPAGE™ precast gel specifications

 

Specifications

Tris-Glycine Gel

Bis-Tris Gel

Concentration

6%, 8%, 10%, 12%, 15%, 4-15%, 4-20%

8%, 10%, 12%, 4-12%

Gel Dimension

Mini gel, 84mm(W)×74mm(H) ×1mm(D)

Cassette Dimension

100mm(W)×89mm(H) ×4.8mm(D)

Gel Thickness

1mm

Stacking Gel

Concentration 4%, 15mm(H)

Well amount and max load

10 wells 50μL, or 15 wells 30μL.

Storage and Shelf Life

4 - 8°C, up to 12 months.

DO NOT FREEZE.

4 - 8°C, up to 12 months.

DO NOT FREEZE.

Shipping

Room temperature or controlled temperature.

Tank Compatibility

AccuPAGE™ is designed to be compatible with all types of MINI-PAGE tank which allows 10cm width gel cassette. Including but not limited to the following models:

1.Bio-Rad Mini-PROTEAN (II/3 /Tetra System) *

2.Hoefer™ Mighty Small (SE250 / SE260 / SE280)

3.Sigma Aldrich Dual Run *

4.Thermofisher/Invitrogen XCell (I, II, & Surelock™ mini-cell) *

5.LONZA PAGEr® Minigel Chamber *

6.Tanon

Running Time

Minimum 20min if Ultra Fast Running Buffer is used.

40 – 60 min by using common buffer.

Application

Native PAGE, denaturing PAGE.

Note: Gel does NOT contain detergent (like SDS).

Running Buffer

Tris-Glycine

MOPS or MES

Package Quantity

2 pcs test pack, 10 pcs, 30 pcs and 50 pcs.

 * Accessory or adaption will be needed. Bio Bench provides the accessories.

Looking for 15 wells precast gel? Contact us at hxinfo@helixarbio.com.

Materials

Plastic cassette

Terms and Conditions

Read before you make an order: Terms and Conditions

Care Instructions

This product is for biotechnology/life science research use only, not for house hold or medical use. Please operate under lab protocol and wear protections, including but not limited to: glasses, lab gloves and lab coat.

View full details

Description

 Documents

Datasheet - AccuPAGE™ Precast Gel

User Guide - AccuPAGE™ Precast Gel

Pay by bank transfer, or any question during purchase? Talk with a representative by email to hxinfo@helixarbio.com.

Bio Bench AccuPAGE™ protein electrophoresis gels are precast polyacrylamide gels that provide large range of molecular weight separation ability with good resolution and sample integrity. AccuPAGE™ provides 2 types of gels, including conventional Tris-Glycine gel and the Bis-Tris gel. These 2 types of gel are both optimized by Bio Bench, in order to provide better performance as well as good stability during long term storage.  AccuPAGE™ is compatible with nearly all of tanks from major biotech providers. Take out from the fridge and open the package and the sealing, then you can operate it under the routine lab instructions.

AccuPAGETris-Gly gel is made of optimized formula from conventional Tris-Glycine gel. It has good stability and long shelf life for up to 12 months. If you lab has been using Tris-Glycine gel, then you can shift to AccuPAGETris-Gly precast gel smooth.

AccuPAGEBis-Tris gel delivers consistent gel performance with a neutral pH environment (pH≈7.0) that minimizes any protein modifications. Bis-Tris can improve the stability of the gel structure during electrophoresis, which will avoid the gel become trapezoid shape and lead to sharp and straight band after staining. Your lab device does not need to be changed to run AccuPAGEBis-Tris gel – only need to remember Bis-Tris gel need to be run in MOPS or MES buffer.


AccuPAGE™ gel separation

 

How to use the AccuPAGE™ precast gel - a quick guide

 

 

 

AccuPAGE™ precast gel family

Gel type

 

 

Running Buffer

 

 

Gel Thickness

 

 

Gel Concentration

 

 

Max Separation

 

 

Recommended Separation

 

 

Well Amount

 

 

Max Sample Load

 

 

Cat. No.

 

 

Tris-Glycine

Tris-Glycine

1mm

6%

40-300kDa

60-200kDa

10

50μL

AP-GPG-610

15

30μL

AP-GPG-615

 

 

8%

25-300kDa

40-200kDa

10

50μL

AP-GPG-810

 

 

15

30μL

AP-GPG-815

 

 

10%

11-230kDa

20-160kDa

10

50μL

AP-GPG-1010

 

 

15

30μL

AP-GPG-1015

 

 

12%

6.5-200kDa

15-85kDa

10

50μL

AP-GPG-1210

 

 

15

30μL

AP-GPG-1215

 

 

15%

5-170kDa

10-50kDa

10

50μL

AP-GPG-1510

 

 

15

30μL

AP-GPG-1515

 

 

4% - 15%

5-270kDa

20-200kDa

10

50μL

AP-GPG-41510

 

 

15

30μL

AP-GPG-41515

 

 

4% - 20%

3.5-300kDa

5-200kDa

10

50μL

AP-GPG-42010

 

 

15

30μL

AP-GPG-42015

 

 

Tris-Bis

MOPS or MES

1mm

8%

8-270kDa

25-245kDa

10

50μL

AP-GPB-810

15

30μL

AP-GPB-815

10%

8-270kDa

11-180kDa

10

50μL

AP-GPB-1010

15

30μL

AP-GPB-1015

12%

3.5-245kDa

11-135kDa

10

50μL

AP-GPB-1210

15

30μL

AP-GPB-1215

4% - 12%

3.5-270kDa

11-245kDa

10

50μL

AP-GPB-41210

15

30μL

AP-GPB-41215

 

 

 

AccuPAGE™ precast gel specifications

 

Specifications

Tris-Glycine Gel

Bis-Tris Gel

Concentration

6%, 8%, 10%, 12%, 15%, 4-15%, 4-20%

8%, 10%, 12%, 4-12%

Gel Dimension

Mini gel, 84mm(W)×74mm(H) ×1mm(D)

Cassette Dimension

100mm(W)×89mm(H) ×4.8mm(D)

Gel Thickness

1mm

Stacking Gel

Concentration 4%, 15mm(H)

Well amount and max load

10 wells 50μL, or 15 wells 30μL.

Storage and Shelf Life

4 - 8°C, up to 12 months.

DO NOT FREEZE.

4 - 8°C, up to 12 months.

DO NOT FREEZE.

Shipping

Room temperature or controlled temperature.

Tank Compatibility

AccuPAGE™ is designed to be compatible with all types of MINI-PAGE tank which allows 10cm width gel cassette. Including but not limited to the following models:

1.Bio-Rad Mini-PROTEAN (II/3 /Tetra System) *

2.Hoefer™ Mighty Small (SE250 / SE260 / SE280)

3.Sigma Aldrich Dual Run *

4.Thermofisher/Invitrogen XCell (I, II, & Surelock™ mini-cell) *

5.LONZA PAGEr® Minigel Chamber *

6.Tanon

Running Time

Minimum 20min if Ultra Fast Running Buffer is used.

40 – 60 min by using common buffer.

Application

Native PAGE, denaturing PAGE.

Note: Gel does NOT contain detergent (like SDS).

Running Buffer

Tris-Glycine

MOPS or MES

Package Quantity

2 pcs test pack, 10 pcs, 30 pcs and 50 pcs.

 * Accessory or adaption will be needed. Bio Bench provides the accessories.

Looking for 15 wells precast gel? Contact us at hxinfo@helixarbio.com.