BIO BENCH
AccuPAGE™ Bis-Tris Precast Gel
AccuPAGE™ Bis-Tris Precast Gel
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| Documents |
Pay by bank transfer, or any question during purchase? Talk with a representative by email to hxinfo@helixarbio.com.
Bio Bench AccuPAGE™ protein electrophoresis gels are precast polyacrylamide gels that provide large range of molecular weight separation ability with good resolution and sample integrity. AccuPAGE™ provides 2 types of gels, including conventional Tris-Glycine gel and the Bis-Tris gel. These 2 types of gel are both optimized by Bio Bench, in order to provide better performance as well as good stability during long term storage. AccuPAGE™ is compatible with nearly all of tanks from major biotech providers. Take out from the fridge and open the package and the sealing, then you can operate it under the routine lab instructions.
AccuPAGE™ Tris-Gly gel is made of optimized formula from conventional Tris-Glycine gel. It has good stability and long shelf life for up to 12 months. If you lab has been using Tris-Glycine gel, then you can shift to AccuPAGE™ Tris-Gly precast gel smooth.
AccuPAGE™ Bis-Tris gel delivers consistent gel performance with a neutral pH environment (pH≈7.0) that minimizes any protein modifications. Bis-Tris can improve the stability of the gel structure during electrophoresis, which will avoid the gel become trapezoid shape and lead to sharp and straight band after staining. Your lab device does not need to be changed to run AccuPAGE™ Bis-Tris gel – only need to remember Bis-Tris gel need to be run in MOPS or MES buffer.
AccuPAGE™ gel separation
How to use the AccuPAGE™ precast gel - a quick guide
AccuPAGE™ precast gel family
|
Gel type
|
Running Buffer
|
Gel Thickness
|
Gel Concentration
|
Max Separation
|
Recommended Separation
|
Well Amount
|
Max Sample Load
|
Cat. No.
|
|
Tris-Glycine |
Tris-Glycine |
1mm |
6% |
40-300kDa |
60-200kDa |
10 |
50μL |
|
|
15 |
30μL |
|
||||||
|
8% |
25-300kDa |
40-200kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
10% |
11-230kDa |
20-160kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
12% |
6.5-200kDa |
15-85kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
15% |
5-170kDa |
10-50kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
4% - 15% |
5-270kDa |
20-200kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
4% - 20% |
3.5-300kDa |
5-200kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
Tris-Bis |
MOPS or MES |
1mm |
8% |
8-270kDa |
25-245kDa |
10 |
50μL |
|
|
15 |
30μL |
|||||||
|
10% |
8-270kDa |
11-180kDa |
10 |
50μL |
||||
|
15 |
30μL |
|||||||
|
12% |
3.5-245kDa |
11-135kDa |
10 |
50μL |
||||
|
15 |
30μL |
|||||||
|
4% - 12% |
3.5-270kDa |
11-245kDa |
10 |
50μL |
||||
|
15 |
30μL |
AccuPAGE™ precast gel specifications
|
Specifications |
Tris-Glycine Gel |
Bis-Tris Gel |
|
Concentration |
6%, 8%, 10%, 12%, 15%, 4-15%, 4-20% |
8%, 10%, 12%, 4-12% |
|
Gel Dimension |
Mini gel, 84mm(W)×74mm(H) ×1mm(D) |
|
|
Cassette Dimension |
100mm(W)×89mm(H) ×4.8mm(D) |
|
|
Gel Thickness |
1mm |
|
|
Stacking Gel |
Concentration 4%, 15mm(H) |
|
|
Well amount and max load |
10 wells 50μL, or 15 wells 30μL. |
|
|
Storage and Shelf Life |
4 - 8°C, up to 12 months. DO NOT FREEZE. |
4 - 8°C, up to 12 months. DO NOT FREEZE. |
|
Shipping |
Room temperature or controlled temperature. |
|
|
Tank Compatibility |
AccuPAGE™ is designed to be compatible with all types of MINI-PAGE tank which allows 10cm width gel cassette. Including but not limited to the following models: 1.Bio-Rad Mini-PROTEAN (II/3 /Tetra System) * 2.Hoefer™ Mighty Small (SE250 / SE260 / SE280) 3.Sigma Aldrich Dual Run * 4.Thermofisher/Invitrogen XCell (I, II, & Surelock™ mini-cell) * 5.LONZA PAGEr® Minigel Chamber * 6.Tanon |
|
|
Running Time |
Minimum 20min if Ultra Fast Running Buffer is used. 40 – 60 min by using common buffer. |
|
|
Application |
Native PAGE, denaturing PAGE. Note: Gel does NOT contain detergent (like SDS). |
|
|
Running Buffer |
Tris-Glycine |
MOPS or MES |
|
Package Quantity |
2 pcs test pack, 10 pcs, 30 pcs and 50 pcs. |
|
* Accessory or adaption will be needed. Bio Bench provides the accessories.
Looking for 15 wells precast gel? Contact us at hxinfo@helixarbio.com.
Materials
Materials
Plastic cassette
Terms and Conditions
Terms and Conditions
Read before you make an order: Terms and Conditions
Care Instructions
Care Instructions
This product is for biotechnology/life science research use only, not for house hold or medical use. Please operate under lab protocol and wear protections, including but not limited to: glasses, lab gloves and lab coat.
Description
| Documents |
Pay by bank transfer, or any question during purchase? Talk with a representative by email to hxinfo@helixarbio.com.
Bio Bench AccuPAGE™ protein electrophoresis gels are precast polyacrylamide gels that provide large range of molecular weight separation ability with good resolution and sample integrity. AccuPAGE™ provides 2 types of gels, including conventional Tris-Glycine gel and the Bis-Tris gel. These 2 types of gel are both optimized by Bio Bench, in order to provide better performance as well as good stability during long term storage. AccuPAGE™ is compatible with nearly all of tanks from major biotech providers. Take out from the fridge and open the package and the sealing, then you can operate it under the routine lab instructions.
AccuPAGE™ Tris-Gly gel is made of optimized formula from conventional Tris-Glycine gel. It has good stability and long shelf life for up to 12 months. If you lab has been using Tris-Glycine gel, then you can shift to AccuPAGE™ Tris-Gly precast gel smooth.
AccuPAGE™ Bis-Tris gel delivers consistent gel performance with a neutral pH environment (pH≈7.0) that minimizes any protein modifications. Bis-Tris can improve the stability of the gel structure during electrophoresis, which will avoid the gel become trapezoid shape and lead to sharp and straight band after staining. Your lab device does not need to be changed to run AccuPAGE™ Bis-Tris gel – only need to remember Bis-Tris gel need to be run in MOPS or MES buffer.
AccuPAGE™ gel separation
How to use the AccuPAGE™ precast gel - a quick guide
AccuPAGE™ precast gel family
|
Gel type
|
Running Buffer
|
Gel Thickness
|
Gel Concentration
|
Max Separation
|
Recommended Separation
|
Well Amount
|
Max Sample Load
|
Cat. No.
|
|
Tris-Glycine |
Tris-Glycine |
1mm |
6% |
40-300kDa |
60-200kDa |
10 |
50μL |
|
|
15 |
30μL |
|
||||||
|
8% |
25-300kDa |
40-200kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
10% |
11-230kDa |
20-160kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
12% |
6.5-200kDa |
15-85kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
15% |
5-170kDa |
10-50kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
4% - 15% |
5-270kDa |
20-200kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
4% - 20% |
3.5-300kDa |
5-200kDa |
10 |
50μL |
|
|||
|
15 |
30μL |
|
||||||
|
Tris-Bis |
MOPS or MES |
1mm |
8% |
8-270kDa |
25-245kDa |
10 |
50μL |
|
|
15 |
30μL |
|||||||
|
10% |
8-270kDa |
11-180kDa |
10 |
50μL |
||||
|
15 |
30μL |
|||||||
|
12% |
3.5-245kDa |
11-135kDa |
10 |
50μL |
||||
|
15 |
30μL |
|||||||
|
4% - 12% |
3.5-270kDa |
11-245kDa |
10 |
50μL |
||||
|
15 |
30μL |
AccuPAGE™ precast gel specifications
|
Specifications |
Tris-Glycine Gel |
Bis-Tris Gel |
|
Concentration |
6%, 8%, 10%, 12%, 15%, 4-15%, 4-20% |
8%, 10%, 12%, 4-12% |
|
Gel Dimension |
Mini gel, 84mm(W)×74mm(H) ×1mm(D) |
|
|
Cassette Dimension |
100mm(W)×89mm(H) ×4.8mm(D) |
|
|
Gel Thickness |
1mm |
|
|
Stacking Gel |
Concentration 4%, 15mm(H) |
|
|
Well amount and max load |
10 wells 50μL, or 15 wells 30μL. |
|
|
Storage and Shelf Life |
4 - 8°C, up to 12 months. DO NOT FREEZE. |
4 - 8°C, up to 12 months. DO NOT FREEZE. |
|
Shipping |
Room temperature or controlled temperature. |
|
|
Tank Compatibility |
AccuPAGE™ is designed to be compatible with all types of MINI-PAGE tank which allows 10cm width gel cassette. Including but not limited to the following models: 1.Bio-Rad Mini-PROTEAN (II/3 /Tetra System) * 2.Hoefer™ Mighty Small (SE250 / SE260 / SE280) 3.Sigma Aldrich Dual Run * 4.Thermofisher/Invitrogen XCell (I, II, & Surelock™ mini-cell) * 5.LONZA PAGEr® Minigel Chamber * 6.Tanon |
|
|
Running Time |
Minimum 20min if Ultra Fast Running Buffer is used. 40 – 60 min by using common buffer. |
|
|
Application |
Native PAGE, denaturing PAGE. Note: Gel does NOT contain detergent (like SDS). |
|
|
Running Buffer |
Tris-Glycine |
MOPS or MES |
|
Package Quantity |
2 pcs test pack, 10 pcs, 30 pcs and 50 pcs. |
|
* Accessory or adaption will be needed. Bio Bench provides the accessories.
Looking for 15 wells precast gel? Contact us at hxinfo@helixarbio.com.